Distinguishing naive- from memory-derived human B cells during acute responses

M Auladell; TH Nguyen; B Garcillán; F Mackay; K Kedzierska; A Fox

Journal article

Distinguishing naive- from memory-derived human B cells during acute responses

M Auladell, TH Nguyen, B Garcillán, F Mackay, K Kedzierska, A Fox

Clinical and Translational Immunology | WILEY | Published : 2019

DOI: 10.1002/cti2.1090

Open access

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Abstract

Objectives: A fundamental question in influenza research is whether antibody titre decline upon successive exposure to variant strains is consequent to recall of cross-reactive memory B cells that competitively inhibit naive B-cell responses. In connection, it is not clear whether naive and memory B cells remain phenotypically distinct acutely after activation such that they may be distinguished ex vivo. Methods: Here, we first compared the capacity of anti-Ig and Toll-like-receptor (TLR) 7/8 and TLR9 agonists (R848 and CpG) to augment human B-cell differentiation induced by IL-21 and sCD40L. The conditions that induced optimal differentiation were then used to compare the post-activation ph..

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University of Melbourne Researchers

Oanh Nguyen Author

Fabienne Mackay Author

Katherine Kedzierska Author

Annette Fox Author

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Funding Acknowledgements

The authors acknowledge Dr Kim Good-Jacobson for her advice. This work was supported by the Australian National Health and Medical Research Council (NHMRC). KK is supported by the NHMRC Program Grant (ID 1071916) and the NHMRC Senior Research Fellowship (ID APP1102792). MA is supported by the Melbourne International Research Scholarship (MIRS) and the Melbourne International Fee Remission Scholarship (MIFRS). This study was funded by the NHMRC Project Grant (ID 1103367). The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health.